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KMID : 0811720100140010051
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Korean Journal of Physiology & Pharmacology
2010 Volume.14 No. 1 p.51 ~ p.57
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Calcium Sensitization Induced by Sodium Fluoride in Permeabilized Rat Mesenteric Arteries
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Yang En-Yue
Cho Joon-Yong
Sohn Uy-Dong
Kim In-Kyeom
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Abstract
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It was hypothesized that NaF induces calcium sensitization in Ca2＋-controlled solution in permeabilized rat mesenteric arteries. Rat mesenteric arteries were permeabilized with ?- escin and subjected to tension measurement. NaF potentiated the concentration-response curves to Ca2＋ (decreased EC50 and increased Emax). Cumulative addition of NaF (4.0, 8.0 and 16 mM) also increased vascular tension in Ca2＋-controlled solution at pCa 7.0 or pCa 6.5, but not at pCa 8.0. NaF-induced vasocontraction and GTP?S-induced vasocontraction were not additive. NaF-induced vasocontraction at pCa 7.0 was inhibited by pretreatment with Rho kinase inhibitors H1152 or Y27632 but not with a MLCK inhibitor ML-7 or a PKC inhibitor Ro31-8220. NaF induces calcium sensitization in a Ca2＋- dependent manner in ?-escin-permeabilized rat mesenteric arteries. These results suggest that NaF is an activator of the Rho kinase signaling pathway during vascular contraction.
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KEYWORD
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Calcium sensitization, Permeabilization, Mesenteric artery, Sodium fluoride, Rho kinase
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